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#
# BioPerl module for Bio::Tools::Run::Phylo::PAML::Evolver
#
# based on the Bio::Tools::Run::Phylo::PAML::Codeml
# by Jason Stajich <jason-at-bioperl.org>
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Albert Vilella <avilella-AT-gmail-DOT-com>
#
# Copyright Albert Vilella
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::Run::Phylo::PAML::Evolver - Wrapper aroud the PAML program evolver
=head1 SYNOPSIS
use Bio::Tools::Run::Phylo::PAML::Evolver;
my $evolver = Bio::Tools::Run::Phylo::PAML::Evolver->new();
# Get a $tree object somehow
$evolver->tree($tree);
# FIXME: evolver generates a tree (first run with option 1 or 2)?
# One or more alns are created
my @alns = $evolver->run();
####
# Or with all the data coming from a previous PAML run
my $parser = Bio::Tools::Phylo::PAML->new
(
-file => "$inputfile",
);
my $result = $parser->next_result();
my $tree = $result->next_tree;
$evolver->tree($tree);
my @codon_freqs = $result->get_CodonFreqs();
$evolver->set_CodonFreqs(\@codon_freqs);
my $val = $evolver->prepare();
# FIXME: something similar for nucleotide frequencies:
# Option (5) Simulate nucleotide data sets (use MCbase.dat)?
# FIXME: something similar for aa parameters:
# Option (7) Simulate amino acid data sets (use MCaa.dat)?
# FIXME: With an initial RootSeq.txt
=head1 DESCRIPTION
This is a wrapper around the evolver program of PAML (Phylogenetic
Analysis by Maximum Likelihood) package of Ziheng Yang. See
http://abacus.gene.ucl.ac.uk/software/paml.html for more information.
This module is more about generating the properl MCmodel.ctl file and
will run the program in a separate temporary directory to avoid
creating temp files all over the place.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/MailList.shtml - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via the
web:
http://bioperl.org/bioperl-bugs/
=head1 AUTHOR - Albert Vilella
Email avilella-AT-gmail-DOT-com
=head1 CONTRIBUTORS
Additional contributors names and emails here
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::Tools::Run::Phylo::PAML::Evolver;
use vars qw(@ISA %VALIDVALUES $MINNAMELEN $PROGRAMNAME $PROGRAM);
use strict;
use Bio::Root::Root;
use Bio::AlignIO;
use Bio::SeqIO;
use Bio::TreeIO;
use Bio::Tools::Run::WrapperBase;
use Bio::Tools::Phylo::PAML;
use Cwd;
@ISA = qw(Bio::Root::Root Bio::Tools::Run::WrapperBase);
=head2 Default Values
Valid and default values for evolver programs are listed below. The
default values are always the first one listed. These descriptions
are essentially lifted from the example MCcodon.dat file and pamlDOC
documentation provided by the author.
Stub:
B<option1> specifies something.
B<option2> specifies something else.
INCOMPLETE DOCUMENTATION OF ALL METHODS
=cut
BEGIN {
$MINNAMELEN = 25;
$PROGRAMNAME = 'evolver' . ($^O =~ /mswin/i ?'.exe':'');
if( defined $ENV{'PAMLDIR'} ) {
$PROGRAM = Bio::Root::IO->catfile($ENV{'PAMLDIR'},$PROGRAMNAME). ($^O =~ /mswin/i ?'.exe':'');;
}
# valid values for parameters, the default one is always
# the first one in the array
# much of the documentation here is lifted directly from the MCcodon.dat
# example file provided with the package
# Evolver calls time for seed: SetSeed(i==-1?(int)time(NULL):i);
my $rand = int(time);
# my $rand = int(rand(999999));
%VALIDVALUES =
(
# FIXME: there should be a 6-7-8 option that fits MCcodon or MCbase or MCaa
'outfmt' => [0,1],
# 0 * 0:paml format (mc.paml); 1:paup format (mc.paup)
# random number seed (odd number)
# FIXME: set seed to null here and ask for it later?
'seed' => "$rand",
# numseq can actually be calculated from the tree external nodes
# nucleotide sites
'nuclsites' => '1000',
# replicates
'replicates' => '1',
# tree length; use -1 if tree has absolute branch lengths
# Note that tree length and branch lengths under the codon model are
# measured by the expected number of nucleotide substitutions per codon
# (see Goldman & Yang 1994). For amino acid models, they are defined as
# the expected number of amino acid changes per amino acid site.
'tree_length' => '1.5',
# omega
# FIXME: if one wants to call for different omegas (NSsites),
# right now it has to be done like:
# $evolver->set_parameter(omega,"3\n0.2\t0.3\t0.5\n0.5\t0.9\t3.2\n");
# 3 * number of site classes, followed by frequencies and omega's.
# 0.6 0.3 0.1 # Freqs
# 0.1 0.8 3.2 # Omegas
'omega' => '0.3',
# kappa
'kappa' => '5',
# FIXME: this only for MCbase.dat ?
# model: 0:JC69, 1:K80, 2:F81, 3:F84, 4:HKY85, 5:T92, 6:TN93, 7:REV
# FIXME: this applies to only some models?
# 10 5 1 2 3 * kappa or rate parameters in model
# FIXME: this applies to only MCbase.dat ?
# 0.5 4 * <alpha> <#categories for discrete gamma>
); # end of validvalues
}
=head2 program_name
Title : program_name
Usage : $factory->program_name()
Function: holds the program name
Returns: string
Args : None
=cut
sub program_name {
return 'evolver';
}
=head2 program_dir
Title : program_dir
Usage : ->program_dir()
Function: returns the program directory, obtained from ENV variable.
Returns: string
Args :
=cut
sub program_dir {
return Bio::Root::IO->catfile($ENV{PAMLDIR}) if $ENV{PAMLDIR};
}
=head2 new
Title : new
Usage : my $obj = Bio::Tools::Run::Phylo::PAML::Evolver->new();
Function: Builds a new Bio::Tools::Run::Phylo::PAML::Evolver object
Returns : Bio::Tools::Run::Phylo::PAML::Evolver
-save_tempfiles => boolean to save the generated tempfiles and
NOT cleanup after onesself (default FALSE)
-tree => the Bio::Tree::TreeI object (FIXME: optional if this is done in a first run)
-params => a hashref of PAML parameters (all passed to set_parameter)
-executable => where the evolver executable resides
See also: L<Bio::Tree::TreeI>
=cut
sub new {
my($class,@args) = @_;
my $self = $class->SUPER::new(@args);
# $self->{'_branchLengths'} = 0;
my ($tree, $st, $params, $exe)
= $self->_rearrange([qw(TREE SAVE_TEMPFILES PARAMS EXECUTABLE)],
@args);
defined $tree && $self->tree($tree);
defined $st && $self->save_tempfiles($st);
defined $exe && $self->executable($exe);
$self->set_default_parameters();
if( defined $params ) {
if( ref($params) !~ /HASH/i ) {
$self->warn("Must provide a valid hash ref for parameter -FLAGS");
} else {
map { $self->set_parameter($_, $$params{$_}) } keys %$params;
}
}
return $self;
}
=head2 prepare
Title : prepare
Usage : my $rundir = $evolver->prepare($aln);
Function: prepare the evolver analysis using the default or updated parameters
the alignment parameter must have been set
Returns : value of rundir
Args : L<Bio::Align::AlignI> object,
L<Bio::Tree::TreeI> object [optional]
=cut
sub prepare {
my ($self,$aln,$tree) = @_;
# FIXME: To consider: to have save_tempfiles always TRUE by default
# or simply never delete
unless ( $self->save_tempfiles ) {
# brush so we don't get plaque buildup ;)
$self->cleanup();
}
$tree = $self->tree unless $tree;
my ($tempdir) = $self->tempdir();
# FIXME:
# If multiple replicates, evolver gives:
# A file with a concatenation of sequential phylips separated by a
# double return which gets correctly parsed by AlignIO next_aln
# Or a concatenation of paup entries with tags separating them which
# wont get correctly parsed with current AlignIO (failed with
# nexus)
# FIXME: To consider: force phylip outfmt and split the files if
# replicates > 1
# if( ! ref($aln) && -e $aln ) {
# $tempseqfile = $aln;
# } else {
# ($tempseqFH,$tempseqfile) = $self->io->tempfile
# ('-dir' => $tempdir,
# UNLINK => ($self->save_tempfiles ? 0 : 1));
# my $alnout = Bio::AlignIO->new('-format' => 'phylip',
# '-fh' => $tempseqFH,
# '-interleaved' => 0,
# '-idlength' => $MINNAMELEN > $aln->maxdisplayname_length() ? $MINNAMELEN : $aln->maxdisplayname_length() +1);
# $alnout->write_aln($aln);
# $alnout->close();
# undef $alnout;
# close($tempseqFH);
# }
# now let's print the MCcodon.dat file.
# many of the these programs are finicky about what the filename is
# and won't even run without the properly named file. Ack
# FIXME: we should do the appropriate here if we are simulating codons, nts o aa.
my $evolver_ctl = "$tempdir/MCcodon.dat";
my $evolverfh;
open($evolverfh, ">$evolver_ctl") or $self->throw("cannot open $evolver_ctl for writing");
# FIXME: params follow an order in the control file, they are not a hash. Do we have an
# clean example of this in bioperl-run?
my %params = $self->get_parameters;
print $evolverfh "$params{outfmt}\n";
print $evolverfh "$params{seed}\n";
# FIXME: call get_leaf_nodes to count only leafs - relates to newick onlyleafids bug
# my $numseq = scalar($tree->get_nodes);
my $numseq = scalar($tree->get_leaf_nodes);
print $evolverfh "$numseq ";
print $evolverfh "$params{nuclsites} ";
print $evolverfh "$params{replicates}\n\n";
print $evolverfh "$params{tree_length}\n";
# FIXME: do #1:#n branch tagging magic here
# FIXME: this pre flush stuff is for appending mode
my $treeout = Bio::TreeIO->new
('-format' => 'newick',
'-fh' => $evolverfh,
-PRE =>'>>',
'-flush',
);
# $treeout->bootstrap_style('nointernalids');
$treeout->write_tree($tree);
# Appending mode to add more control file contents here
open($evolverfh, ">>$evolver_ctl") or $self->throw("cannot open $evolver_ctl for writing");
print $evolverfh "\n$params{omega}\n";
print $evolverfh "$params{kappa}\n";
# Print codon freqs here or defaults (below)
my @codon_freqs = $self->get_CodonFreqs();
foreach my $firstbase (@codon_freqs) {
foreach my $element (@$firstbase) {
print $evolverfh " $element";
}
print $evolverfh "\n";
}
# FIXME: codon freqs or nt freqs should always come from an object?
# Silly printing the default codonfreqs in the default
# MCcodon.dat provided by PAML
unless (@codon_freqs) {
print $evolverfh
"0.00983798 0.01745548 0.00222048 0.01443315\n",
"0.00844604 0.01498576 0.00190632 0.01239105\n",
"0.01064012 0.01887870 0 0\n",
"0.00469486 0.00833007 0 0.00688776\n",
"0.01592816 0.02826125 0.00359507 0.02336796\n",
"0.01367453 0.02426265 0.00308642 0.02006170\n",
"0.01722686 0.03056552 0.00388819 0.02527326\n",
"0.00760121 0.01348678 0.00171563 0.01115161\n",
"0.01574077 0.02792876 0.00355278 0.02309304\n",
"0.01351366 0.02397721 0.00305010 0.01982568\n",
"0.01702419 0.03020593 0.00384245 0.02497593\n",
"0.00751178 0.01332811 0.00169545 0.01102042\n",
"0.02525082 0.04480239 0.00569924 0.03704508\n",
"0.02167816 0.03846344 0.00489288 0.03180369\n",
"0.02730964 0.04845534 0.00616393 0.04006555\n",
"0.01205015 0.02138052 0.00271978 0.01767859\n";
}
print $evolverfh "\n// end of file.\n";
close($evolverfh);
# FIXME: what do we return in prepare?
# return
}
=head2 run
Title : run
Usage : my ($rc,$parser) = $evolver->run();
Function: run the evolver analysis using the default or updated parameters
the alignment parameter must have been set
Returns : Return code, L<Bio::Tools::Phylo::PAML>
Args : L<Bio::Align::AlignI> object,
L<Bio::Tree::TreeI> object [optional]
=cut
sub run {
my $self = shift;
# FIXME: We should look for the stuff we prepared in the prepare method here
my $rc = (1);
{
my $exit_status;
my ($tmpdir) = $self->tempdir();
chdir($tmpdir);
my $evolverexe = $self->executable();
$self->throw("unable to find or run executable for 'evolver'") unless $evolverexe && -e $evolverexe && -x _;
open(RUN, "$evolverexe 6 MCcodon.dat |") or $self->throw("Cannot open exe $evolverexe");
my @output = <RUN>;
$exit_status = close(RUN);
$self->error_string(join('',@output));
if ( (grep { /\berr(or)?: /io } @output) || !$exit_status) {
$self->warn("There was an error - see error_string for the program output");
$rc = 0;
}
# FIXME - hardcoded mc.paml
unless ($self->indel) {
my $in = Bio::AlignIO->new('-file' => "$tmpdir/mc.paml",
'-format' => 'phylip');
my $aln = $in->next_aln();
$self->alignment($aln);
}
}
return $rc;
}
=head2 error_string
Title : error_string
Usage : $obj->error_string($newval)
Function: Where the output from the last analysis run is stored.
Returns : value of error_string
Args : newvalue (optional)
=cut
sub error_string{
my ($self,$value) = @_;
if( defined $value) {
$self->{'error_string'} = $value;
}
return $self->{'error_string'};
}
=head2 alignment
Title : alignment
Usage : $evolver->align($aln);
Function: Get/Set the L<Bio::Align::AlignI> object
Returns : L<Bio::Align::AlignI> object
Args : [optional] L<Bio::Align::AlignI>
Comment : We could potentially add support for running directly on a file
but we shall keep it simple
See also: L<Bio::SimpleAlign>
=cut
sub alignment{
my ($self,$aln) = @_;
if( defined $aln ) {
if( -e $aln ) {
$self->{'_alignment'} = $aln;
} elsif( !ref($aln) || ! $aln->isa('Bio::Align::AlignI') ) {
$self->warn("Must specify a valid Bio::Align::AlignI object to the alignment function not $aln");
return undef;
} else {
$self->{'_alignment'} = $aln;
}
}
return $self->{'_alignment'};
}
=head2 tree
Title : tree
Usage : $evolver->tree($tree, %params);
Function: Get/Set the L<Bio::Tree::TreeI> object
Returns : L<Bio::Tree::TreeI>
Args : [optional] $tree => L<Bio::Tree::TreeI>,
[optional] %parameters => hash of tree-specific parameters:
branchLengths: 0, 1 or 2
out
Comment : We could potentially add support for running directly on a file
but we shall keep it simple
See also: L<Bio::Tree::Tree>
=cut
sub tree {
my ($self, $tree, %params) = @_;
if( defined $tree ) {
if( ! ref($tree) || ! $tree->isa('Bio::Tree::TreeI') ) {
$self->warn("Must specify a valid Bio::Tree::TreeI object to the alignment function");
}
$self->{'_tree'} = $tree;
# FIXME: I think we dont need this in Evolver
# if ( defined $params{'_branchLengths'} ) {
# my $ubl = $params{'_branchLengths'};
# if ($ubl !~ m/^(0|1|2)$/) {
# $self->throw("The branchLengths parameter to tree() must be 0 (ignore), 1 (initial values) or 2 (fixed values) only");
# }
# $self->{'_branchLengths'} = $ubl;
# }
}
return $self->{'_tree'};
}
=head2 get_parameters
Title : get_parameters
Usage : my %params = $self->get_parameters();
Function: returns the list of parameters as a hash
Returns : associative array keyed on parameter names
Args : none
=cut
sub get_parameters{
my ($self) = @_;
# we're returning a copy of this
return %{ $self->{'_evolverparams'} };
}
=head2 set_parameter
Title : set_parameter
Usage : $evolver->set_parameter($param,$val);
Function: Sets a evolver parameter, will be validated against
the valid values as set in the %VALIDVALUES class variable.
The checks can be ignored if one turns off param checks like this:
$evolver->no_param_checks(1)
Returns : boolean if set was success, if verbose is set to -1
then no warning will be reported
Args : $param => name of the parameter
$value => value to set the parameter to
See also: L<no_param_checks()>
=cut
sub set_parameter{
my ($self,$param,$value) = @_;
unless ($self->{'no_param_checks'} == 1) {
if ( ! defined $VALIDVALUES{$param} ) {
$self->warn("unknown parameter $param will not be set unless you force by setting no_param_checks to true");
return 0;
}
if ( ref( $VALIDVALUES{$param}) =~ /ARRAY/i &&
scalar @{$VALIDVALUES{$param}} > 0 ) {
unless ( grep { $value eq $_ } @{ $VALIDVALUES{$param} } ) {
$self->warn("parameter $param specified value $value is not recognized, please see the documentation and the code for this module or set the no_param_checks to a true value");
return 0;
}
}
}
$self->{'_evolverparams'}->{$param} = $value;
return 1;
}
=head2 set_default_parameters
Title : set_default_parameters
Usage : $evolver->set_default_parameters(0);
Function: (Re)set the default parameters from the defaults
(the first value in each array in the
%VALIDVALUES class variable)
Returns : none
Args : boolean: keep existing parameter values
=cut
sub set_default_parameters{
my ($self,$keepold) = @_;
$keepold = 0 unless defined $keepold;
while( my ($param,$val) = each %VALIDVALUES ) {
# skip if we want to keep old values and it is already set
next if( defined $self->{'_evolverparams'}->{$param} && $keepold);
if(ref($val)=~/ARRAY/i ) {
$self->{'_evolverparams'}->{$param} = $val->[0];
} else {
$self->{'_evolverparams'}->{$param} = $val;
}
}
}
=head1 Bio::Tools::Run::WrapperBase methods
=cut
=head2 no_param_checks
Title : no_param_checks
Usage : $obj->no_param_checks($newval)
Function: Boolean flag as to whether or not we should
trust the sanity checks for parameter values
Returns : value of no_param_checks
Args : newvalue (optional)
=cut
sub no_param_checks{
my ($self,$value) = @_;
if( defined $value) {
$self->{'no_param_checks'} = $value;
}
return $self->{'no_param_checks'};
}
=head2 set_CodonFreqs
Title : set_CodonFreqs
Usage : $obj->set_CodonFreqs($newval)
Function: Get/Set the Codon Frequence table
Returns : value of set_CodonFreqs (a scalar)
Args : on set, new value (a scalar or undef, optional)
=cut
sub set_CodonFreqs{
my $self = shift;
return $self->{'_codonfreqs'} = shift if @_;
return $self->{'_codonfreqs'};
}
=head2 get_CodonFreqs
Title : get_CodonFreqs
Usage : my @codon_freqs = $evolver->get_CodonFreqs()
Function: Get the Codon freqs
Returns : Array
Args : none
=cut
sub get_CodonFreqs{
my ($self) = @_;
return @{$self->{'_codonfreqs'} || []};
}
=head2 save_tempfiles
Title : save_tempfiles
Usage : $obj->save_tempfiles($newval)
Function:
Returns : value of save_tempfiles
Args : newvalue (optional)
=cut
=head2 outfile_name
Title : outfile_name
Usage : my $outfile = $evolver->outfile_name();
Function: Get/Set the name of the output file for this run
(if you wanted to do something special)
Returns : string
Args : [optional] string to set value to
=cut
=head2 tempdir
Title : tempdir
Usage : my $tmpdir = $self->tempdir();
Function: Retrieve a temporary directory name (which is created)
Returns : string which is the name of the temporary directory
Args : none
=cut
=head2 cleanup
Title : cleanup
Usage : $evolver->cleanup();
Function: Will cleanup the tempdir directory after a PAML run
Returns : none
Args : none
=cut
=head2 io
Title : io
Usage : $obj->io($newval)
Function: Gets a L<Bio::Root::IO> object
Returns : L<Bio::Root::IO>
Args : none
=cut
sub DESTROY {
my $self= shift;
unless ( $self->save_tempfiles ) {
$self->cleanup();
}
$self->SUPER::DESTROY();
}
=head2 indel
Title : indel
Usage : $obj->indel($newval)
Function: this is only useful if using evolver_indel instead of main
evolver package:
Exploring the Relationship between Sequence Similarity and
Accurate Phylogenetic Trees Brandi L. Cantarel, Hilary
G. Morrison and William Pearson
Example :
Returns : value of indel (a scalar)
Args : on set, new value (a scalar or undef, optional)
=cut
sub indel{
my $self = shift;
return $self->{'indel'} = shift if @_;
return $self->{'indel'};
}
1;
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